Journal: Therapeutic Advances in Medical Oncology

Article Title: Mismatch repair protein imbalance in head and neck squamous cell carcinoma: associations with clinical features and survival

doi: 10.1177/17588359251408596

Figure Lengend Snippet: Correlation between MMR protein expression and immune cell subsets in HNSCC. (a–f) Correlation of MSH2, MSH6, PMS2, MLH1, MSH2/MSH6, and PMS2/MLH1 ratios with immune cell subsets. (g) Diagram of correlation patterns among MMR proteins. (h) Correlation between MSH2 expression and CD8+ T cells in nontumor tissues. (i) Correlation between MSH6 expression and CD4+ T cells in tumor tissues. (j) Correlation between PMS2/MLH1 ratio expression and CD4+ T-cell in tumor tissues. HNSCC, head and neck squamous cell carcinoma; MMR, mismatch repair.

Article Snippet: Briefly, MSH2 (ZSGB-BIO ® ; ZA-0622, 1:2000), MSH6 (Roche Diagnostics GmbH ® ; SP93, ready-to-use), PMS2 (Dako ® ; EP51, ready-to-use), MLH1 (Dako ® ; ES05, ready-to-use), CD4 (Abcam; ab133616, 1:2000), CD8 (SinoBiological; 10980-R081, 1:500), and Foxp3 (Abcam; ab191416, 1:1000) antibodies were used to immunohistochemically stain the TMA slides, with brown staining considered as positive immunoreaction and blue staining considered as negative immunoreaction (nuclear, cytoplasm, or membrane, based on cells of interest).

Techniques: Expressing

Journal: Biomolecules

Article Title: Human Mutant Dynactin Subunit 1 Causes Profound Motor Neuron Disease Consistent with Possible Mechanisms Involving Axonopathy, Mitochondriopathy, Protein Nitration, and T-Cell-Mediated Cytolysis

doi: 10.3390/biom15121637

Figure Lengend Snippet: T cells invade the spinal cord parenchyma of mutant-DCTN1 tg mice. ( A ) Localization of Fas-positive cells (thin arrows) in ventral horn of wildtype DCTN1 transgenic mouse. Scale bar (same for ( B )) = 40 µm. ( B ) Fas-positive cells (thin arrows) in the ventral horn of mutant DCTN1 tg mice appear more numerous than in wildtype tg mice. Inset is dashed square showing Fas-positive cells in the vicinity of a motor neuron. Inset scale bar = 12 µm. ( C ) Fas-positive cells (thin arrows) near degenerating motor neurons (asterisk) in mutant-DCTN1 mice. Scale bar = 7 µm. ( D ) Box plot showing the mean Fas-positive cell number (with IQR and 5–95 percentile whiskers, n = 6/group) in the ventral horn of age-matched non-tg, human wildtype DCTN1 (p150) and human mutant DCTN1 (p150) at 8 months of age. ( E ) Infiltrated CD8-positive cells (arrows) in the ventral horn of mutant DCTN1 mice. CD8 immunoreactivity is also present in the surrounding white matter (wm). Scale bar = 12 µm. ( F ) Focal accumulations of CD8 immunoreactivity were seen in the ventral root exit zones in the ventral funiculus of mutant DCTN1 mice. Scale bar = 15 µm.

Article Snippet: The sections were blocked and permeabilized (1 h) in 10% normal goat serum (NGS) with 0.4% Triton-x 100 and then incubated (4 °C) in primary IgG antibodies overnight: rabbit monoclonal anti-cleaved caspase-8 (1:500, D5B2), rabbit monoclonal anti-cleaved caspase-3 (1:4000, D3E9), rabbit polyclonal anti-SOD2 (1:2000), rabbit polyclonal anti-Parkin (1:500), rabbit polyclonal anti-mouse TNF-α (Chemicon, St. Louis, MO, USA, 1:500), rabbit monoclonal anti-IL9 (Abcam, Cambridge, MA, USA, 1:500), monoclonal anti-nitrated Hsp90 (1:1000), a mouse monoclonal anti-Fas (BD Transduction Laboratories, Franklin Lakes, NJ, USA, 1:500), and a rabbit polyclonal antibody to CD8 (Proteintech, 1:500).

Techniques: Mutagenesis, Transgenic Assay